Plant Tissue Cult. 9(1) : 17-23, 1999 (June)

Clonal Propagation of Emblica officinalis Through In vitro Culture

M. M. Rahman, P. K. Roy¹, M. A. Mannan and Shyamal K. Roy

Department of Botany, Jahangirnagar University, Savar, Dhaka, Bangladesh

Key words: Regeneration, Multiple shoots, Emblica officinalis

Abastract

For in vitro multiple shoot regeneration, shoot tips and nodal segments of five-year-old tree of Emblica officinalis were used as explants. Multiple shoot induction was found best in B5 medium supplemented with 1.0 mg/1 BAP and 0.25 mg/1 NAA. Addition of coconut milk (CM) in the medium enhanced the number of shoots per culture. Excised shoots rooted when they were transferred to half-strength of MS medium containing 0.5 mg/1 each of NAA, IAA and IBA. The regenerated plantlets were successfully acclimatized and established in soil. About 80% of plantlets survived under ex vitro conditions.

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