Plant Tissue Cult. 9(1) : 11-16, 1999 (June)

In vitro Plant Regeneration from Leaf Explants of Soybean {Glycine max (L.) Merr.}

A. Settu and B. D. Ranjithakumari

Department of Plant Science, School of Life Sciences, Bharathidasan University, Thiruchirappalli-620024, India

Key words: Glycine max, Plant regeneration, Leaf explants

Abastract

Plant regeneration from leaf explants of soybean has been developed. Callus induction and shoot regeneration at various frequencies were observed using different concentrations and combinations of growth regulators. The highest percentage of callus induction and proliferation was observed in the medium containing NAA (2.0 mg/1) and Kn (0.5 mg/1). The maximum percentage of shoot bud formation was obtained on MS medium fortified with BAP (3.0 mg/1) and NAA (0.5 mg/1). The regenerated shoots were transferred to shoot elongation medium containing 2.0 mg/1 of GA₃ and 0.2 mg/1 of BAP. The elongated shoots developed roots on a medium containing IBA (2.0 mg/1) and Kn (0.2 mg/1). The rooted plants were transferred to plastic container for hardening.

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