Plant Tissue Cult. 9(1) : 1-9, 1999 (June)

Effect of Preincubation of Tubers on In vitro Culture of Three Potato Varieties

S. A. K. U. Khan¹ and M. G. Rabbani

Department of Horticulture, Bangladesh Agricultural University, Mymensingha-2202, Bangladesh

Key words: Potato, Preincubation, In vitro culture, Growth rate

Abastract

Effects of dark and light preincubation of tubers on in vitro performance of three potato varieties were investigated using tuber sprouts as explants. The highest percentage of cultures (83.59) survived when sprouts from dark preincubated tubers were used. Days to new shoot initiation, height of the plantlets, number of roots, average root length, number of nodes, number of leaves, growth rate and vigour of the plantlets were significantly influenced by preicubation on tubers in the dark. Among the varieties, Lalpakri showed better performance followed by HPSII-67 and Cardinal in respect of different characters of in vitro grown plantlets. Significant interaction between preincubation of tubers and potato varieties was observed in respect of different in vitro characteristics of potato (except per cent survivability, days to new shooting, days to new rooting and vigour of the plantslets). Better performance was observed in the treatment combination of sprouts from dark preincubated tubers in variety Lalpakri.

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