Plant Tissue Cult. 8(1) : 173-175, 1998 (December)

Short Communications

In vitro Genotype-Phytohormone Interaction in Chickpea (Cicer arietinum L.)

R. Islam, H. Farooqui and S. Raizuddin

Centre for Advanced Molecular Biology, University of Punjab, Lahore, Pakistan

Key words: Chickpea, Growth regulators, Regression, Stability

Abastract

Genetic transformation and creation of an elite germplasm calls for an efficient plant regeneration system. Chickpea (Cicer arietinum), a major grain legume is susceptible to virulent strains of Agrobacterium (Islam et al. 1994) but due to lack of a high frequency regeneration system has deterred the production of transgenic plants. In vitro plant regeneration in chickpea from different explants has been reported but different genotypes showed various response under different phytohormone concentrations and combinations (Islam et al. 1995). Genotypic variation under different hormonal variation has also been reported in other legumes (Lutova and Zabelina 1988). Hence it has been a common experience with high yielding chickpea that the in vitro plant regeneration ability of this crop is highly influenced among other factors by the phytohormone concentrations and combinations. In the present investigation and attempt has been made to study quantitatively the genotype phytohormone interaction to select suitable genotype for tissue culture research.

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