Plant Tissue Cult. 8(1) : 157-164, 1998 (December)

In vitro Shoot Regeneration and Ex vitro Rooting in Chrysanthemum morifolium Ramat

M. I. Hoque, Mustari Taslim Jahan and R. H. Sarker

Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Key words: Shoot regeneration, Rooting, Chrysanthemum

Abastract

In vitro large scale shoot regeneration was tried from the shoot tip and nodal explants of five varieties of chrysanthemum. Different concentration and combinations of BAP, Kn, NAA and IAA were used in MS and modified MS media for the above purpose. Best response towards multiple shoot regeneration was obtained from the nodal explants on MS medium containing 1.0 mg/1 BAP and 0.5 mg/1 NAA. In the above combination nodal explant produced 12-15 initial shoot buds whereas shoot tip explants produced 8-10 shoot buds. Rooting at the base of the regenerated shoots was tried under both in vitro and ex vitro conditions. In case of in vitro rooting best response was obtained when shoots were cultured on half the strength of MS medium supplemented with 0.2 mg/1 IBA. On the other hand under ex vitro condition best rooting was observed when the shoots were treated with 0.2 mg/1 IBA foe one hour prior to their transplantation on to sand. Both in vitro and ex vitro rooted plantlets were successfully transplanted in the soil.

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