Plant Tissue Cult. 8(1) : 101-107, 1998 (December)

Rapid Multiplication of Eucalyptus camaldulensis by in vitro Culture of Nodal Explants

P. Venkatachalam¹, N. Geetha1 and A. Saravanababu²

Bidhan Chandra Krishi Viswavidyalaya, Mohanpur ? 741 252, West Bengal, India

Key words: Eucalyptus camaldulensis, Micropropagation, Plantlet regeneration, Nodal explats

Abastract

Shoot bud multiplication has been achieved in Eucalyptus camaldulensis using nodal explants from 15-year-old elite trees growing in field. Of the two cytokinins tested, BAP was very effective for induction of shoots and the best concentration was 1.5 mg/1. Results showed that addition of NAA at 0.5 mg/1 induced maximum number of shoot buds. In both Kn and ADS supplemented media induced multiple shoot formation, although at a smaller frequency than BAP took place. The regenerated shoots were successfully rooted. Rooting was best on half-strength MS medium containing IBA. The regenerated plants have been successfully acclimatized and transferred to soil. About 45% of the plants have survived under ex vitro condition. This system could be beneficial for mass propagation of selected elite clones.

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