An efficient callus induction and plant regeneration system has been developed using three local soybean (Glycine max L.) varieties. All the varieties showed good callusing (78.30 - 88.80%) from shoot tip (ST) in MS + 3.0 mg/l 2,4-D + 1.0 mg/l BAP. Best callus induction and embryo formation were recorded in T5 for BS-6 as 88.80 and 81.20%, respectively from ST. In case of cotyledonary node (CN) the maximum callus induction (82.40%) and embryo formation (74.20%) were recorded also in T5 for BS-6. Highest frequency (79.40%) of plant regeneration was recorded where ST were used and cultured in MS supplemented with 2.5 mg/l BAP + 1.0 mg/l NAA as well as 76.30% from CN in BS-6. The length of shoot was observed 5.32 and 4.62 cm, respectively from ST and CN for BS-6 with the same medium composition. It was observed that half strength of MS + 2.0 mg/l IBA showed best rooting (9.04). Among the genotypes BS-6 proved to be best explants than ST that exhibited better performance on callus induction and green plant regeneration for all parameters.
Copies of the Journal of
Plant Tissue Culture & Biotechnology (PTC&B) ISSN : 1817-3721 (Half yearly Journal of BAPTC&B) Indexed by Biological Abstracts, BIOSIS Previews, Elsevier Bibliographic Databases Available from the General Secretary,
Bangladesh Association for Plant Tissue Culture & Biotechnology (BAPTC&B)
C/o, Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh.