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Plant Tissue Cult. 5(1) : 21-25, 1995



Direct Organogenesis in Cultured Hypocotyl Explants of Aegel marmelos Corr.

M. Hossain, R. Islam, A. Islam1 and O.I. Joarder

Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh

Key words: In vitro culture, Adventitious shoots, Aegel marmelos

Abastract

A procedure for adventitious shoot regeneration from hypocotyls explants of Aegel marmelos using benzyladenine (BA) and indole-3-acetic acid (IAA) is described. Excised hypocotyls segments from seedlings grown on hormone-free MS medium and 0.8% agar were used. When the medium contained 1.5 mg/1 BA and 0.2 mg/1 IAA, 79% of the explants produced shoots. The number of shoot regenerants from hypocotyls excised from 4-5-week-old-seedlings was greater than those older than five weeks. Regenerated shoots were cultured on MS medium containing 0.1 mg/1 BA and they rooted on half strength MS medium containing 1.0 mg/1 indole-3-butyric acid (IBA).

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ISSN : 1817-3721
(Half yearly Journal of BAPTC&B)
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Plant Tissue Culture & Biotechnology (BAPTC&B)

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